View the calculated relative level of gene expression for the replicate group that is associated with the test sample. View the calculated ΔΔC T value added to or subtracted from the F-Sigma value calculated for the replicate group associated with the reference sample. View the calculated ΔΔC T value for the replicate group associated with the reference sample. View the calculated F-Factor for the replicate group associated with the reference sample. (Quant Space value) View the standard error of the sample-replicate-group-level normalized quantity values. For singleplex experiments, the calculation combines the plate-level C q value variation between the target and the endogenous control. For multiplex experiments, the calculation is at the well level. Note: The ∆C T/∆C RT SE value is calculated differently for multiplex and singleplex experiments. For more information, see Cq/Quant space. You can change the Result Detail setting (Cq or Quant Space) from the View The RQ Plot allows you to view the RQ computations in either Cq Space (using ΔΔCt analysis) or Quant Space (incorporating efficiency correction using quantities). You can view the plot as the following graph types: linear, log10, Ln, log2. RQ vs BioGroup – (present when the plot displays results by Biogroups) – Groups the relative quantification (RQ) values by biological replicate group. RQ vs Sample (present when the plot displays results by Samples) – Groups the relative quantification (RQ) values by sample.
RQ vs Target – Groups the relative quantification (RQ) values by target. The RQ (Relative Quantitation) Plot displays the results of the relative quantification calculations in the gene expression profile.
0 kommentar(er)
